3D4/21-CAS9 cell line

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3D4/21-CAS9 Order

Catalog#

YC-C041-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

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Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

3D4/21-CAS9

Morphology

Macrophage-like, adherent

Passage ratio

1:2~1:3

Species

Pig

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

85%RPMI-1640+10%FBS+1%Glutamax+1%SodiumPyruvate+1%NEAA+1%Hepes+2.2g/ml Glucose

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the 3D4/21 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a 3D4/21-CAS9 cell line that can maintain long-term, stable and high expression.

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

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